Pharmaceutical Regulatory Affairs: Open Access

Hideharu Shintani

Publications

• Review Article
  Inactivation of Endotoxin and Lipid A by Nitrogen Gas Plasma Exposure Running title: Inactivation Endotoxin and lipid A
  Author(s): Hideharu ShintaniHideharu Shintani
  
  The gases plasma from several different gases has shown a sporicidal activity. From these gases, nitrogen gas was most difficult to produce atomic nitrogen radicals. However, these radicals have a high energy, indicating that nitrogen gas plasma could be used to sterilize microorganisms and inactivate endotoxins and lipid A. The sterilization mechanism of nitrogen gas plasma may be the synergistic effect of free radicals such as (OH radical and NO radical) and metastables (N2 or O2 metastable). Thus, the target microorganisms such as bioburden were damaged by degradation, which resulted in death. The biological indicator BI used in this study was Geobacillus stearothermophilus ATCC 7953 at an initial population of 106 CFU/carrier. Sterility assurance level (SAL) of 10-6 was confirmed by using the BI. In addition endotoxins were successfully inactivated by nitrogen gas plasma expossure.. Read More»
  DOI: 10.4172/2167-7689.1000129

  Abstract PDF