Journal of Tissue Science and Engineering

Bernd Rolauffs

Publications

• Research Article
  The TGF-β1-Induced Expression of Matrix Metalloproteinases in Mesenchymal Stromal Cells is Influenced by Type of Substrate
  Author(s): Katrin Warstat, Tino Felka, Falk Mittag, Torsten Kluba, Bernd Rolauffs, Melanie L. Hart, Wilhelm K. Aicher and Gerd KleinKatrin Warstat, Tino Felka, Falk Mittag, Torsten Kluba, Bernd Rolauffs, Melanie L. Hart, Wilhelm K. Aicher and Gerd Klein
  
  Transforming growth factor (TGF)-?1 activates the expression of matrix metalloproteinases (MMPs) in fibroblasts. Attachment of these cells to laminin-111 further raises the TGF-?1-induced expression of MMP-3 and MMP-10. Mesenchymal stromal cells (MSC) attach to a variety of extracellular matrix proteins during development and wound healing. We therefore investigated the TGF-?1-regulated expression of MMPs in MSC upon attachment to laminin-111 and type I collagen. The expression of MMPs was determined by quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. The TGF-?1 signalling pathways were investigated by immunoblot and by pharmacological blocking of Smad2, MEK/ERK and p38MAPK activities. Overall, TGF-?1 significantly activated the expression of mRNA encoding MMP-3 (p=0.05), MMP-13 (p=0.05) and TIMP-1 (p=0.01) in MSC. In.. Read More»
  DOI: 10.4172/2157-7552.1000108

  Abstract PDF