Veterinary Science & Technology

ISSN: 2157-7579

Open Access

Aslam A


  • Research Article
    Pathological Investigation and Molecular Detection of Avian Pathogenic E. coli Serogroups in Broiler Birds
    Author(s): Azeem Riaz M, Aslam A, Rehman M and Yaqub TAzeem Riaz M, Aslam A, Rehman M and Yaqub T

    The objective of the present study the rfb gene clusters in avian pathogenic E. coli cardinal serotypes O1, O2 and O78 strains and to develop a multiplex polymerase chain reaction method for serotyping of the O-antigens. The multiplex polymerase chain reaction method was used for the identification of serotypes of APEC. The second part of the study was to study the pathological lesions caused by most prevalent sero group in experimentally infected broiler chicks. A total of 100 tissue samples (50 lungs and livers each) were collected from colibacillosis suspected broiler birds and subjected to isolation and identification of E. coli by conventional methods. Multiplex PCR was used for confirmation of three serogroups i.e., O1, O2 and O78. We found more O2 33% than O1 8% and O78 zero percent. These results suggested the prevalence of O2 sero group in our study.. Read More»
    DOI: 10.4172/2157-7579.1000373

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