Urinary tract infection remains one of the most common infections, both in the community and in the hospital. The ever-increasing number of patients presenting with urinary tract infection and the number of relapse cases of urinary tract infections after treatment in rivers state, Nigeria. The prevalence and trend of urinary tract infection, isolation and identification of bacteria isolates from urine samples were examined and the determination of the antibiogram of isolates among patients attending hospitals in rivers state. A total of sixty (60) early morning midstream urine samples were collected from different hospital patients who are sexually active between the ages of 18years and 45years. 30 urine samples were obtained from the University Of Port Harcourt Teaching Hospital (UPTH) and 30 urine samples also O.B. Lulu-Briggs Health Centre respectively and were macroscopically and microscopically examined. Out of the 60 samples, 40 of them were collected from females while 20 of them from males. The bacteria isolates identified during this study include Escherichia coli, Klebsiella sp., Staphylococcus aureus, Streptococcus sp., Proteus sp. and Pseudomonas aeruginosa. The number and percentage frequency of occurrence of bacteria isolated from the urine samples that had growth are Escherichia coli 17 (40.4%), Klebsiella sp. 6 (14%), Staphylococcus aureus 9 (21.4%), Streptococcus sp. 3 (7.14%), Proteus sp. 4 (9.5%), and Pseudomonas aeruginosa 4 (7.14%). For samples collected at Lulu Briggs Health Centre, the bacterial isolates obtained are Escherichia coli 7 (38.8%), Klebsiella sp. 2 (11%), Staphylococcus aureus 5 (27.7%), Streptococcus sp. 2 (11.1%), Proteus sp. 1 (5.5%), and Pseudomonas aeruginosa 1 (5.5%). For samples collected at University of Port Harcourt Teaching Hospital (UPTH), the bacterial isolates obtained are Escherichia coli 10 (41.7%), Klebsiella sp. 4 (16.6%), Staphylococcus aureus 4 (16.6%), Streptococcus sp. 1 (4.1%), Proteus sp. 3 (12.5%),
and Pseudomonas aeruginosa 2 (8.3%).