GET THE APP

..

Metabolomics:Open Access

ISSN: 2153-0769

Open Access

NMR-Based Metabolomic Analyses

Abstract

Fujian Wilson*

Silica nanoparticles are increasingly used in the biomedical fields due to their excellent solubility, high stability and favorable biocompatibility. However, despite being considered of low genotoxicity, their bio-related adverse effects have attracted particular concern from both the scientific field and the public. In this study, human cervical adenocarcinoma cells (HeLa line) were exposed to 0.01 or 1.0 mg/mL of hydrophilic silica nanoparticles. The 1H NMR spectroscopy coupled with multivariate statistical analysis were used to characterize the metabolic variations of intracellular metabolites and the compositional changes of the corresponding culture media. At the early stage of silica nanoparticles-exposure, no obvious dose–effect of HeLa cell metabolome was observed, which implied that cellular stress-response regulated the metabolic variations of HeLa cell.

PDF

Share this article

Google Scholar citation report
Citations: 895

Metabolomics:Open Access received 895 citations as per Google Scholar report

Metabolomics:Open Access peer review process verified at publons

Indexed In

 
arrow_upward arrow_upward