The rapid and sensitive detection of V. cholerae bacteria in vegetable and environmental water samples is crucial for ensuring public health and preventing the spread of cholera. In this study, we developed an ultrasensitive voltammetric genosensor for the rapid detection of V. cholerae. The genosensor utilized specific DNA probes designed to target the unique DNA sequences of V. cholerae, enabling highly selective and sensitive detection. The performance of the genosensor was evaluated using vegetable and environmental water samples spiked with varying concentrations of V. cholerae. The results demonstrated the ultrasensitive detection capability of the genosensor, with a detection limit below the recommended threshold for safe consumption. The developed genosensor offers a promising approach for the rapid and reliable detection of V. cholerae in vegetable and environmental water samples, aiding in the prevention and control of cholera outbreaks.
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