Chan Li, Xianlei Li, Shuyi Li, Yuhua Weng, Kaiyuan Wang, Tingbin Zhang, Shizhu Chen, Xuexia Lu, Yonggang Jiang, Jing Xu and Xingjie Liang
Herein we report development and validation of the method for evaluating the encapsulation efficiency of a micelle-based nanosystem composed of irinotecan hydrochloride (CPT-11) and an amphiphilic molecule DSPEmPEG2000. The results showed that the centrifugation method can be used for separation of free drug, a critical step in measuring encapsulation efficiency, and the EE of three batches of CPT-11/DSPE-mPEG2000 micelles was 90.0% ± 1.0%. The results also indicated that the conditions used in the process have to be optimized to acquire reliable data.
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