P Lavudu, AP Rani, CB Sekaran, KS Kumar, A Ramesh
A simple and sensitive visible spectrophotometric method has been developed for the determination of pramipexole dihydrochloride (PPD). The method is based on the condensation of the drug with acetyl acetone and formaldehyde producing a yellow colored Hantzsch condensation product having maximum absorption at 455 nm. The method is optimized for pH, concentration of reagents (acetyl acetone and formaldehyde) required, temperature and heating time. Beer’s law was obeyed in the concentration range of 5–150 μg mL-1. Molar absorptivity, Sandell’s sensitivity, limit of detection and limit of quantification were calculated and were found to be 5.23 x 103 L mole-1 cm-1, 0.0849 μg cm-2, 0.449 μg mL-1, and 1.362 μg mL-1, respectively. The method was applied to different tablet dosage forms containing PPD. The results of the proposed method were compared with the reported UV spectrophotometric method. The new method was found to be simple, accurate (t-test), and reproducible (F-test).
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