The resistance mechanism of Escherichia coli induced by ampicillin in laboratory

Joint Event on 14^th International Conference on Microbial Interactions & Microbial Ecology & 11^th Edition of International Conference on Advances in Microbiology and Public Health

August 19-20, 2019 Vienna, Austria

Mengchen Li

Zhengzhou University, China

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Pathogenic Escherichia coli often causes diarrhea, sepsis and other clinical symptoms, and is still one of the main intestinal pathogens affecting human and animal health. The aim of this study was to compare the differences in non-synonymous single nucleotide polymorphisms (non-SNP) between drug-resistant strains and sensitive strains, and to understand the function of related genes and the metabolic pathways involved. We simulated the process of clinical antibiotics in organisms by using the method of ampicillin laboratory induction and explored the relationship between the degree of drug resistance and the mutation site. This study provides a basis for the early detection of drug-resistant strains, the rational and effective application of antibiotics and a new idea for solving the problem of E.coli resistance. Combined mutations of ftsI and envZ genes were found to induce higher resistance. Mutations in the frdD, OmpD, acrB and VgrG genes may also be linked to antibiotic resistance in β-lactamide antibiotic.

Biography :

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