The outcome of Minibiobank under adverse conditions in Iraq

International Conference and Exhibition on Tissue Preservation & Bio-banking

July 20-22, 2015 Barcelona, Spain

Mohemid Maddallah Mohammed Al-Jebouri

Posters-Accepted Abstracts: J Tissue Sci Eng

Abstract :

Background: The finding of human umbilical cord blood as one of the most likely sources of hematopoietic stem cells offers a less invasive alternative for the need of hematopoietic stem cell transplantation. Due to the once-in-alifetime chance of collecting it, an optimum cryopreservation method that can preserve the life and function of the cells contained is critically needed. Methods: Until now, slow-cooling has been the routine method of cryopreservation; however, rapid-cooling offersa simple, efficient, and harmless method for preserving the life and function of the desired cells. Therefore, this study was conducted to compare the effectiveness of slow- and rapid-cooling to preserve umbilical cord blood of mono nucleated cells suspected of containing hematopoietic stem cells. The parameters used in this study were differences in cell viability, malondialdehyde content, and apoptosis level. The identification of hematopoietic stemcells themselves was carried out by enumerating CD34+ in a flow cytometer. Results: Our results showed that mononucleated cell viability after rapid-cooling (91.9%) was significantly higher than that after slow-cooling (75.5%), with a p value = 0.003. Interestingly, the malondialdehyde level in the mono nucleated cell population after rapid-cooling (56.45 μM) was also significantly higher than that after slow cooling (33.25 μM), with a p value < 0.001. The apoptosis level in rapid-cooling population (5.18%) was not significantly different from that of the mononucleated cell population that underwent slow-cooling (3.81%), with ap value = 0.138. However, CD34+ enumeration was much higher in the population that underwent slow-cooling (23.32 cell/μl) than in the one that underwent rapid-cooling (2.47 cell/μl), with ap value = 0.001. Conclusions: Rapid-cooling is a potential cryopreservation method to be used to preserve the umbilical cordblood of mononucleated cells, although further optimization of the number of CD34+ cells after rapid-cooling is critically needed.