Mshelia B M, Adeshina G O, Onaolapo J A, Sirajo U, Aliyu B and Zaharaddin M
Federal University Birnin Kebbi, Nigeria
Posters & Accepted Abstracts: J Med Microb Diagn
This study was aimed to determine the antibacterial activity of honey and/or lemon juice on strains of Streptococcus pneumoniae and Streptococcus pyogenes from respiratory tract infections. Clinical isolates were collected from Ahmadu Bello University Teaching Hospital (ABUTH), Zaria and Ahmadu Bello University Health Services (ABUHS) Samaru campus, Zaria. The isolates were characterized by standard microbiological procedures. Antibacterial activities of the honey and lemon juice, as well as that of some standard antibiotic formulations were assayed using agar well diffusion and broth dilution method. Minimum inhibitory and bactericidal concentrations were carried out. Rate of kill was also carried out to determine the death/survival rate of the bacterial isolates after exposure to the agents. Noticeable variations in the antibacterial activity of the agents were observed. Thus, inhibition zones (mm) ranging from 10-22 (100% honey), 14-29 (100% lemon) and 20-29 (honey and lemon juice mixture) were obtained. However, minimum inhibitory concentrations (μg/ml) range between 1.95-125 (ceftriaxone), 1.56-NI (gentamicin), 31.5-NI (amoxicillin/clavulanic acid), 0.98-62.5 (levofloxacin), 50.0-NI (azithromycin), 20.0-75.0 (100% v/v honey), 22.5-47.5 (100% v/v lemon juice) and 17.5-25.0 (honey and lemon juice mixture). However, for the rate of kill, honey and lemon juice mixture, lemon juice affected complete killing at 120 minutes; while, ceftriaxone, levofloxacin and honey produced complete killing at 1440 minutes. Therefore, from the findings, honey and lemon juice mixture, lemon juice, levofloxacin, ceftriaxone and gentamicin had higher antibacterial activity than azithromycin, amoxicillin-clavulanic acid and honey. However, for the statistical analysis, at p≥0.05, there is significant difference between honey and lemon juice mixture and honey. In conclusion, the bacterial isolates were more susceptible to honey and lemon juice mixture, lemon juice, levofloxacin, ceftriaxone and gentamicin; but less susceptible to azithromycin, amoxicillin-clavulanic acid and honey. Excellent bactericidal activity was observed with honey and lemon juice mixture, lemon juice compared to the honey alone. The findings in this research therefore provides scientific basis to the use of honey and lemon juice as an alternative medicine by the populace in the treatment of respiratory tract infections.
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