Role of glypican-3 immunocytochemistry in differentiating hepatocellular carcinoma from metastatic carcinoma of the liver utilizing fine needle aspiration cytology

7^th Global Summit on Cancer Therapy

October 05-07, 2015 Dubai, UAE

Mohammed Zaakouk

Cairo University, Egypt

Posters-Accepted Abstracts: J Cancer Sci Ther

Abstract :

Purpose: Evaluation of the sensitivity and specificity of glypican3 (GPC3) in differentiating hepatocellular carcinoma (HCC) from metastatic carcinomas of the liver in cell block material. Patients & Methods: Sixty cell blocks were prepared from liver FNAs performed in the radio-diagnosis Department, National Cancer Institute, in the period (Aug, 2011 to May, 2012). FNAs were performed in the presence of the cytopathologist for on-site evaluation of the aspirated material and preparation of cell blocks. Cases diagnosed as hepatocellular carcinoma or metastatic carcinoma was included in the study. Cell block sections were stained with anti GPC-3. Sensitivity, specificity and positive and negative predictive values of GPC3 were calculated. The final diagnosis was based on the triple approach of clinical data, radiological findings as well as cytomorphologic features aided by GPC-3 results. Results: 70% of cases were diagnosed as HCC and 30% as metastatic carcinomas. 95.2% of HCC cases expressed GPC3. Poorly differentiated cases showed the highest GPC3 sensitivity (100%) followed by moderately differentiated cases (96.5%) and while well differentiated cases expressed GPC3 in 90% of cases. 83.3% of metastatic carcinomas were negative for GPC3. In this study, sensitivity of GPC-3 in HCC was 95.2%, specificity was 83.3%, positive and negative predictive values were 93% and 88.2% respectively and total accuracy was 91.7%. Conclusion: Immunocytochemical staining for GPC3 in cell block material is a highly sensitive and specific method capable of distinguishing HCC from the vast majority of metastatic carcinomas of the liver.

Biography :

Email: Zaakouk@gmail.com