Proteomic analysis of membrane proteins in spheroid 3-dimensional bullet of human umbilical cord blood mesenchymal stem cells

International Conference on Tissue Science & Engineering

October 1-3, 2012 DoubleTree by Hilton Chicago-North Shore, USA

Dong Hyon Kim, Ji Hyun Kim, Yoon Sun Yang, Wonil Oh and Jong Wook Chang

Posters: J Tissue Sci Eng

Abstract :

R ecently, aggregating of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) improved therapeutic efficacy in animal model. In order to elucidate the biochemical property of aggregated hUCB-MSCs as a spheroid 3-dimensional bullet, membrane proteins were analyzed by 2D-PAGE and identified by LC-MS. Expression level of total 18 membrane proteins were changed reproducibly compared to that of monolayer hUCB-MSC. Among these, several proteins were selected to understand molecular mechanism of MSC aggregation for improving therapeutic efficacy