Abolfazl Akbarzadeh, Amir Ahmad Khandaghi and Soodabeh Davaran
Accepted Abstracts: J Cancer Sci Ther
In the field of cancer therapy, magnetic nanoparticles modified with biocompatible copolymer are promising vehicles for the delivery of hydrophobic drugs such as doxorubicin. The major aim of this effort was to evaluate whether doxorubicin-loaded magnetic nanoparticles improved the anti-tumor effect of free doxorubicin in lung cancer cells. A series of PLGA-PEG triblock copolymer were synthesized by ring-opening polymerization of D, L-lactide and glycolide with polyethylene glycol (PEG 4000 ) as an initiator. The bulk properties of these copolymer were characterized using 1 H nuclear magnetic resonance spectroscopy, gel permeation chromatography, Fourier transform infrared spectroscopy, and differential scanning calorimetry. Three doxorubicin- loaded magnetic nanoparticles were synthesized by the double emulsion method (w/o/w). Cytotoxic assays were evaluated in lung carcinoma (A549) cells treated by the MTT assay technique. In addition, the particles were characterized by X-ray powder diffraction, scanning electron microscopy, fourier transform infrared spectroscopy, and vibrating sample magnetometry. The anti-proliferative effect of doxorubicin appeared much earlier when the drug was encapsulated in magnetic nanoparticles than when it was free. Doxorubicin-loaded magnetic nanoparticles significantly enhanced the decrease in IC50 rate. The in vitro cytotoxicity test showed that the Fe 3 O 4 -PLGA-PEG 4000 magnetic nanoparticles had no cytotoxicity and were biocompatible. The chemotherapeutic effect of free doxorubicin on lung cancer cells is improved by its encapsulation in modified magnetic nanoparticles. This approach has the prospective to overcome some major limitations of conventional chemotherapy and may be a promising strategy for future applications in lung cancer therapy. Keywords: Magnetic Nanoparticles, Doxorubicin, Drug Delivery, Lung Cancer.