Non cryogenic alternatives for biobanking cells and germplasm

International Conference and Exhibition on Tissue Preservation & Bio-banking

July 20-22, 2015 Barcelona, Spain

Pasqualino Loi

Posters-Accepted Abstracts: J Tissue Sci Eng

Abstract :

Current cryopreservation techniques are robust, but storage is expensive, needs specialized storage facilities and continuous supply of liquid nitrogen. The author demonstrated for the first time that lyophilized somatic cells direct embryonic development upon injection into enucleated oocytes. These findings have been a stepping-stone for a further demonstration that lyophilized cord blood cells were capable of forming colonies upon rehydration. This talk will focus on preliminary data generated in my laboratory on the use of recombinants Late Embryogenesis Abundant (LEA) proteins - physiologically expressed in seeds during maturation - to confer desiccation tolerance in somatic cells.