MicroRNA-30a-5p inhibits gallbladder cancer cell proliferation, migration and metastasis by targeting E2F7

32^nd Euro Congress on Cancer Science & Therapy

March 07-08, 2019 | Barcelona, Spain

Yuan-Yuan Ye

Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

Gallbladder carcinoma (GBC), the most common malignant tumor of the bile duct, is highly aggressive and has a poor prognosis. MicroRNA-30a-5p (miR-30a-5p) is an important tumor suppressor that participates in many aspects of carcinogenesis and cancer development. However, the role of miR-30a-5p in GBC development remains to be determined, as do the mechanisms underlying its effects in GBC. Using samples collected from 42 subjects with gallbladder carcinoma (GBC), we showed decreased miR-30a-5p expression in the primary lesions versus non-tumor adjacent tissues (NATs). Decreased miR-30a-5p was associated with shorter disease-free survival (DFS) and overall survival (OS). Inhibiting miR-30a-5p expression in two representative GBC cell lines (GBC-SD and NOZ) increased cell proliferation, migration, invasiveness, as well as -catenin nuclear translocation, vice versa. In nude mice, NOZ cells transfected with miR-30a-5p mimics grew slower (vs. miR-NC) upon subcutaneous inoculation, and had lower rate of hepatic metastasis upon spleen inoculation. Dual luciferase assay confirmed that E2F transcription factor 7 (E2F7) was a direct target of miR-30a-5p and antagonized the effects induced by miR-30a-5p down-regulation in GBC cells. MiR- 30a-5p attenuates the EMT and metastasis in GBC cells by targeting E2F7 suggesting miR-30a-5p is a tumor suppressor that may serve as a novel potential prognostic biomarker or molecular therapeutic target for GBC.

Biography :

E-mail: yeyuanyuan19891028@126.com