In Silico Test, Sintysis and Analog Anti Folat Activity Test in the HeLa Cell as Anti Servics Cancer

8^th Euro Global Summit on Cancer Therapy

November 03-05, 2015 Valencia, Spain

Ahmad Fatih, Ihsan Adi, Chusnul Chotimah, Aris Setiyo and Aulia Agiel

Posters-Accepted Abstracts: J Cancer Sci Ther

Abstract :

Indonesia, each year approximately 15,000 new cases of cervical cancer and 7,500 of them lead to death. In cancer cells was found that excess folic acid needs. Have been many analog of folic acid (antifolate) is used as the analog cancer chemotherapy but still have considerable side effects. So it is necessary to modify the folic acid molecule to produce another analog that is expected to provide an optimal activity but have low toxicity. Folic acid modification begins with drafting a compound where folic acid is added the groups which have lipophilic properties, electronic and steric certain. Then tested in silico to predict bond strength analogous to receptors. Then do the synthesis of these compounds with Schotten-Baumann method and the its analog results tested the activity in cervical cancer cells with in vitro assays in HeLa cells that compared to the positive control (methotrexate) with MTT assay method. Results from in silico test shows modified compounds have lower scores rerank (potent). Results synthesis proved by infra red spectrophotometric and h-nmr spectrophotometric test. the compounds synthesized tested with MTT assay test that compared with methotrexate at a level of 250 ppm of obstacles 8.1 (compounds synthesized) and 83.1 (methotrexate). This research resulted in 2-N- (benzoyl) �folate acid as antifolate that proved to be more potent. Keywords : Cervics Cancer, Folat Acid, HeLa Cell

Biography :

Email: adiprabowo56@gmail.com