Arika Wahyuningsih
Sunan Ampel Islamic University, Indonesia
Posters & Accepted Abstracts: J Mol Genet Med
HER2I655V detection method for breast cancer frozen tissue origin have been successfully developed. However, these methods have still used sampling of breast cancer that is impassive (making the patient's pain due to tissue sampling procedure through biopsy). Therefore, the present study developed a method of detection of HER2I655V using buccal cells as a source of genetic material that is safe and does not create additional pain to the patient, besides taking samples of DNA from buccal cells more applicable for genetic screening program HER2I655V involving many samples. The purpose of this study was to isolate DNA from buccal cells and perform HER2I655V SNP detection using the AS-PCR to determine the usefulness of the buccal cells as an alternative to DNA samples of non-impassive. The result of this study is successfully performed with the size of AA genotype 142 bp and genotype GG 168 bp. Confirmation test was conducted with 5 samples from buccal cells known genotype AA. The preparation success was indicated by no contamination in the test sample AS-PCR with NTC added in the test.
Email: arika_timi@yahoo.com
Molecular and Genetic Medicine received 3919 citations as per Google Scholar report