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Fragment gene preparation with Allele-Specific Polymerase Chain Reaction (AS-PCR) method for standard marker of HER2I655V
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Molecular and Genetic Medicine

ISSN: 1747-0862

Open Access

Fragment gene preparation with Allele-Specific Polymerase Chain Reaction (AS-PCR) method for standard marker of HER2I655V


2nd World Congress on Molecular Genetics and Gene Therapy

July 03-04, 2017 Bangkok, Thailand

Arika Wahyuningsih

Sunan Ampel Islamic University, Indonesia

Posters & Accepted Abstracts: J Mol Genet Med

Abstract :

HER2I655V detection method for breast cancer frozen tissue origin have been successfully developed. However, these methods have still used sampling of breast cancer that is impassive (making the patient's pain due to tissue sampling procedure through biopsy). Therefore, the present study developed a method of detection of HER2I655V using buccal cells as a source of genetic material that is safe and does not create additional pain to the patient, besides taking samples of DNA from buccal cells more applicable for genetic screening program HER2I655V involving many samples. The purpose of this study was to isolate DNA from buccal cells and perform HER2I655V SNP detection using the AS-PCR to determine the usefulness of the buccal cells as an alternative to DNA samples of non-impassive. The result of this study is successfully performed with the size of AA genotype 142 bp and genotype GG 168 bp. Confirmation test was conducted with 5 samples from buccal cells known genotype AA. The preparation success was indicated by no contamination in the test sample AS-PCR with NTC added in the test.

Biography :

Email: arika_timi@yahoo.com

Google Scholar citation report
Citations: 3919

Molecular and Genetic Medicine received 3919 citations as per Google Scholar report

Molecular and Genetic Medicine peer review process verified at publons

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