Ex vivo platforms to evaluate CD4 and CD8 T cell immune responses to pathogens and candidate vaccines

Global Veterinary Microbiology and Veterinary Medicine Summit

October 17-18, 2016 Chicago, USA

William C Davis, Gaber S Abdellrazeq, Mahmoud M Elnaggar, John P Bannantine, Kun Taek Park, Victoria Mack and Lindsay M Fry

Washington State University, USA
Alexandria University, Egypt
United States Department of Agriculture-Agricultural Research Service, USA
Seoul National University, South Korea

Posters & Accepted Abstracts: J Veterinar Sci Techno

Abstract :

Ex vivo models were developed to study the functional activity of effector T cells proliferating in response to presentation of candidate vaccines, processed and presented by monocyte derived dendritic cells (MoDC). PBMC from a steer vaccinated with a Mycobacterium paratuberculosis (Map) deletion mutant (Map/relA) were used to demonstrate the potential of the models to study the effector T cell response to a live vaccine and a candidate Map major membrane protein (MMP). PBMC and CD14 depleted PBMC were used to show CD4 and CD8 T cells proliferated, when stimulated with Map/relA showing blood DC actually presented the antigens. MoDC pulsed with Map/relA were used to demonstrate a CD4 and CD8 T cell response. MMP was used to demonstrate a component of the response was directed towards MMP, a candidate for a peptide-based vaccine. CD8 T cells proliferating in response to antigens presented by MoDC pulsed with Map/relA were used to demonstrate their ability to kill intracellular Map. The models obviate many of the difficulties in assessing the potential efficacy of vaccines before testing in the field.

Biography :

Email: davisw@vetmed.wsu.edu