Cunzhi Han, Yan Wang, Lili Du, Jin Shi, Jiexian Jing, Xianwen Zhao, Lixia Wang and Xing Wang
Accepted Abstracts: J Cancer Sci Ther
Aim:To explore the correlation between the protein expression and polymorphism of leptin receptor (LEPR) gene and the occurrence, development and pathologic characteristics of breast cancer. The protein expression of LEPR and its Gln223Arg polymorphism in pre-surgery breast tumor tissues of 150 patients, in benign tumor tissues of 80 patients and in normal paracancerous tissues of 50 patients and in anti-coagulant cubital vein blood of 128 healthy subjects were examined using immunohistochemical staining and PCR-RFLP, respectively. The positive rate of LEPR expression in breast tumor tissues was 70.67%, significantly higher than that of 56.25% in benign breast tissues and that of 44.0% in normal paracancerous breast tissues (P<0.039 and P<0.005, respectively). In breast cancer patients, LEPR gene Gln223Arg genotype of GG ,GA and AA expressions were 70.0%,16.67% and 13.33% ,which were significantly different from those of the benign breast lesions 82.50%,13.75%, 3.75%, in paracancerous normal breast tissues 82.0%, 14.0%, 4.0% and the health control of 82.81%, 14.85% and 2.34%( P=0.005); alleles genes (G and A) were 78.33% and 21.60% in breast cancer patients,which were significantly different from those of the benign breast lesions 89.37% and 10.63%, in paracancerous normal breast tissues 89.42%, 10.58 and in anti-coagulant cubital vein blood of health control of 90.23%, 9.77% (P=0.001). In addition, LEPR expression was correlated neither with menopausal status, histological type, tumor size, tumor grade and metastasis status (P>0.05), nor the expression of estrogen receptor (ER) and progesterone receptor (PR) (P>0.05).However, the positive rate (87.8%) of LEPR expression in patients with lymph node metastasis was significantly higher than that of 60.2% in patients with no lymph node metastasis (P=0.02). Non-conditional logistic regression analysis indicated that the high LEPR expression, the LEPR gene Gln223Arg polymorphism and high WHR were correlated with the occurrence of breast cancer (OR=5.06, 95% CI: 1.35~10..28, P=0.002; OR=3.82, 95% CI: 1.28~18.13, P=0.012; OR = 3.62, 95%CI: 1.32~9.97, P=0.017). High expression of LEPR, LEPR gene Gln223Arg polymorphism and enhanced WHR are related with the high risk for breast cancer Keywords: Breast cancer, Leptin receptor gene, Gln223Arg polymorphism.