COMPERATIVE ANALYSIS OF MICROORGANISMS AND HEAVY METAL CONTAMINATION OF PERIWINKLE (Tympanutonus fuscatus and Pachymalania aurita) IN AKWA IBOM STATE

47^th World Congress on Microbiology

September 10-11, 2018 | London, UK

EKPO, M. A. and B ATOYEBI

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Microbiological and heavy metal concentrations of periwinkle (Tympanotonus fuscatus and Pachymalania aurita) harvested from three aquatic ecosystems in Akwa Ibom State were determined using standard microbiological and chemical techniques. The antibiotic susceptibility profiles of the isolates were determined using disc diffusion technique. The total heterotrophic bacterial counts and total coliform counts of the periwinkle samples ranged from 3.4x107�±0.07 to 4.1x107�±0.29 cfu/g and 2.8x105�±0.33 to 4.1x105�±0.38 cfu/g, respectively. The total fungal and total vibrio counts ranged from 1.4x105�±0.44 to 2.2x105�±0.25 cfu/g and 9.2x104�±2.18 to 1.0x105�±2.64 cfu/g, respectively. The faecal coliform and total salmonella-shigella counts ranged from 1.6x105�±1.93 to 2.4x105�±0.14 cfu/g and 1.9x105�±2.13 to 2.5x105�±0.22 cfu/g, respectively. Eleven bacterial genera comprising Bacillus, Salmonella, Escherichia, Enterobacter, Pseudomonas, Shigella, Staphylococccus, Vibrio, Serratia, Proteus and Streptococcus were isolated from the samples, while the fungal isolates were Aspergillus, Mucor, Penicillum, Rhizopus and Candida species. The bacterial isolates were highly resistant to Amoxicillin/Clavulanates and Cefuroxime, while high sensitivities to Gentamicin were observed among E. coli, Staphylococcus spp. and Pseudomonas spp. The concentrations of lead and copper in the periwinkle samples ranged from 6.076�±0.004 to 9.158�±0.05mg/l and 6.621.049�±0.006 to 103.850�±0.099mg/l, respectively, while the cadmium and chromium concentrations in the samples ranged from 0.641�±0.980 to 1.054�±1.441 and 0.050�±1.681 to 12.615�±2.051mg/kg, respectively. The concentrations of mercury in the samples ranged from 0 to 1.291�±0.185 mg/kg. There was a significant correlation (p<0.05) in the microbial loads and heavy metals concentration of the samples. The microbial counts and bioconcentration of heavy metals in the unprocessed periwinkle obtained in this study were beyond specified standard limits by International Commission of Microbiological Specification for Food and Federal Environmental Protection Agency. Consequently, the periwinkle should be properly processed before consumption

Biography :

E-mail: emacopron@yahoo.com