Caveolin-1 K.O. mice exposed to a high fat diet exhibit a lipotoxic milieu but less beta pancreatic damage compared with wild type mice

29^th International Congress on Prevention of Diabetes and Complications

September 27-28, 2018 | Berlin, Germany

Sergio Wehinger, Olinda Nunez, Paola Llanos and Paloma Lillo

Universidad de Talca, Chile
Universidad de Chile, Chile

Scientific Tracks Abstracts: J Diabetic Complications Med

Abstract :

Background: Deleterious effects of high levels of free fatty acids lead to a phenomenon known as �??lipotoxicity�?�, associated with insulin resistance and beta pancreatic cell damage, key events in development of type 2 diabetes mellitus. Lipotoxicity has been associated with cellular oxidative stress and beta-cell apoptosis. Caveolin-1 is a membrane protein that has been associated with many cellular functions as cell signaling regulation and apoptosis, and it is normally present in beta pancreatic cells. We previously reported that the expression of the membrane protein Caveolin-1 promotes free fatty acids-induced apoptosis in vitro in a mouse beta cell line. Remains to be elucidated if this phenomenon is relevant in vivo Methods: We used C57BL6J mice lacking expression of Caveolin-1 (Caveolin-1 K.O. mice). We evaluated the free fatty acids and triglycerides levels in blood in fasting conditions, oral glucose tolerance test (OTTG), carbonylated proteins in serum and C-peptide in wild type (WT) and Caveolin-1 K.O. and wild type mice exposed to a high fat diet for three months. Also, the presence of apoptosis was evaluated by TUNEL staining in beta pancreatic islets. Results: We found that Caveolin-1 K.O. mice fed with high fat diet showed higher levels of triglycerides, cholesterol, free fatty acids and carbonylated proteins, although also a better response OGTT and C-peptide levels. Islets from K.O. mice showed lower levels of apoptosis. Conclusion: Although K.O. mice showed a lipotoxic profile, our results suggest that their pancreatic islets were more resistant to the high fat diet deleterious effects over beta cells. Recent Publications 1. Zagmut S., Leiva E., Mujica V., Wehinger S., (2016). Protective Effect of Propolis Extract on Pancreatic Cell under Oxidative Stress in vitro. Journal of Food Nutrition and Research. Vol. 4, No. 6, 2016, pp 400-407 2. Wehinger S.; Ortiz R.; Díaz M.I.; Aguirre A; Valenzuela M.; Llanos P; Mc Master C.; Leyton L; Quest A.F.G. (2015) Phosphorylation of caveolin-1 on tyrosine-14 induced by ROS enhances palmitate-induced death of beta- pancreatic cells, Biochim. Biophys. Acta. 1852:693-708 3. Leiva E; Mujica V.; Orrego, R; Wehinger S.; Soto A; Icaza, G; Vásquez, M.; Diaz, L; Andrews M.; Arredondo M. (2014) Subjects with Impaired Fasting Glucose: Evolution in a Period of 6 Years. ournal of Diabetes Research. 5 pages. 4. Núñez-Wehinger, S; Ortiz, R J; Díaz, N; Díaz, J; Lobos-González, L; Quest, A F G. (2014). Caveolin-1 in cell migration and metastasis. Current molecular medicine 14:255-274 5. Quest, A F G; Lobos-González, L; Nuñez, S; Sanhueza, C; Fernández, J-G; Aguirre, A; Rodríguez, D; Leyton, L; Torres, V. (2013). The caveolin-1 connection to cell death and survival. Current molecular medicine. 13(2):266-81.

Biography :

Wehinger has completed his PhD in Biomedical Sciences from University of Chile in 2013 and actually is the Director of Magister in Biomedical Sciences of University of Talca. He is currently investigating the molecular mechanisms involved in the cellular failure of the beta pancreatic islets, which is induced by elevated free fatty acids and oxidative stress levels, to elucidate how to prevent these processes.

E-mail: stevefoxster@gmail.com