Badmus J A, Ekpo O E, Ahmed H A, Meyer M and Hiss D C
University of the Western Cape, South Africa
Ladoke Akintola University of Technology, Nigeria
Posters & Accepted Abstracts: J Cancer Sci Ther
Holamine and funtumine cytotoxicity and cell cycle arrest had been previously reported. The present study evaluates the apoptotic effects, reactive oxygen species (ROS) induction, mitochondrial depolarization, caspase-3 induction, F-actin perturbation of the two steroidal alkaloids in cancer cell lines (HT-29, MCF-7 and HeLa) and inhibition of topoisomerase-1. Apoptotic effect was studied by flow cytometry using the APOPercentageTM dye, Annexin-V/PI stain and induction of caspase-3 using the Caspase-3/7 Glo assay kit. Furthermore, ROS generation, mitochondrial perturbation, F-actin and topoisomerase-1 relaxation assay were evaluated following standard procedure. The two steroidal alkaloids were found to be more cytotoxic to cancerous cell than non-cancerous fibroblast. The cytotoxicity of the two alkaloids was mediated through induction of apoptosis. The apoptotic activation was found to be triggered by the activation of caspase-3, induced ROS, mitochondrial toxicity, F-actin disorganization and topoisomerase-1 inhibition. The study relates the cytotoxicity activities of the two compounds for the stimulation of apoptosis pathway through induction of ROS, mitochondrial depolarization, F-actin disorganization and topoisomearase-1 inhibition.
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