Abstract

Researches in the field of cellular and molecular mechanisms of cell variability, based on phenotypic interactions, remain one of the most central and promising problems in modern biology. The current paper proposes a possible model for studying "variability windows" and cell phenotype reprogramming using different somatic cell types (epithelial and mesenchymal cells) as well as different cell culture systems - 2D monolayer, 3D single spheroids and microtissues accompanied with up-to-date live time-lapse microscopy analysis.