The separation of DNA molecules according to their size represents a fundamental bioanalytical procedure. Here, we report the development of a chip-sized device, composed of micrometric-sized fence structures manufactured in a microchannel, for the separation of large DNA molecules (more than 10 kbp) based on the principle steric exclusion chromatography (SEC). In order to achieve separation, two approaches were used: first, the DNA samples were concentrated immediately before separation using nanoslit structures, in order to improve the resolution. Second, a theoretical separation model based on SEC was established and applied in order to predict the optimal voltage range for separation. In this study, we performed the separation of λ DNA (48.5 kbp) and T4 DNA (166 kbp) using the current SEC-based microchip.
Scientific Tracks Abstracts: Journal of Clinical Case Reports
Scientific Tracks Abstracts: Journal of Clinical Case Reports
Posters & Accepted Abstracts: Journal of Advanced Practices in Nursing
Posters & Accepted Abstracts: Journal of Advanced Practices in Nursing
Scientific Tracks Abstracts: Journal of Advanced Practices in Nursing
Scientific Tracks Abstracts: Journal of Advanced Practices in Nursing
Scientific Tracks Abstracts: Journal of Advanced Practices in Nursing
Scientific Tracks Abstracts: Journal of Advanced Practices in Nursing
Posters & Accepted Abstracts: Journal of Advanced Practices in Nursing
Posters & Accepted Abstracts: Journal of Advanced Practices in Nursing
Clinical and Medical Case Reports received 53 citations as per Google Scholar report
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