The biocompatibility of fluorescent nanomaterials and their interaction with the biological system and cellular uptake kinetic is an important aspect to understand the development of new generation nanoprobe. The foremost objective of this study is to evaluate the paramagnetic aqueous soluble 3-Mercaptopropionic acid capped ZnSe: Mn2+/Eu2+ and ZnSe: Eu2+, Mn2+/ZnS core/shell quantum dots (QDs) for the fabrication of clinically diagnostic nanoprobe. Primarily, QDs were synthesized via high-temperature organometallic methods and performed their morphological and physicochemical analyses. The QDs demonstrated an ultra-small zinc-blend cubic crystal structure with an elliptical shape and an average diameter of 4 nm. Under UV light, they exhibited two different characteristics emissions from Mn2+ at 584 nm, and Eu2+ around 495 nm. The toxicity and uptake evaluation of QDs was performed in Raw 264.7 macrophages at different time intervals and temperatures. The QDs showed mild time-dependent toxicity at a concentration of 1mg/μl. For the uptake analysis, cells were examined by enhanced dark-field hyperspectral microscopy (CytoViva) and transmission electron microscopy (TEM) techniques. The findings demonstrate that cells exhibited no significant uptake at ice; however, at 37ºC, they showed time-dependent internalization and subsequent gradual appearance of clusters in the intracellular regions. Moreover, the TEM study shows that the 3-MPA QDs are adhered to the cell and subsequently internalized as single particles or aggregates. The time-dependent cellular uptake and substantial cluster formation suggest that the 3-MPA QDs can be effectively used for diagnostic and theranostic purposes.
Case Report: Clinical and Medical Case Reports
Case Report: Clinical and Medical Case Reports
Case Report: Clinical and Medical Case Reports
Case Report: Clinical and Medical Case Reports
Case Report: Clinical and Medical Case Reports
Case Report: Clinical and Medical Case Reports
Case Report: Clinical and Medical Case Reports
Case Report: Clinical and Medical Case Reports
Editorial: Clinical and Medical Case Reports
Editorial: Clinical and Medical Case Reports
Posters & Accepted Abstracts: Journal of Clinical Case Reports
Posters & Accepted Abstracts: Journal of Clinical Case Reports
Scientific Tracks Abstracts: Journal of Clinical Case Reports
Scientific Tracks Abstracts: Journal of Clinical Case Reports
Posters & Accepted Abstracts: Journal of AIDS & Clinical Research
Posters & Accepted Abstracts: Journal of AIDS & Clinical Research
Posters & Accepted Abstracts: Journal of Advanced Practices in Nursing
Posters & Accepted Abstracts: Journal of Advanced Practices in Nursing
Clinical and Medical Case Reports received 53 citations as per Google Scholar report
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