Tissue innervation is a complex process controlled by the expression profile of signaling molecules secreted by tissue resident cells that dictate the growth and guidance of axons. Sensory innervation is part of the neural network of bone tissue with a spatio-temporal occurrence defined during bone development. However, the current understanding of the mechanisms regulating the map of sensory innervation in bone tissue is still limited. Here, we have demonstrated that the differentiation of human mesenchymal stem cells into osteoblasts results in a marked alteration of their capacity to promote axonal growth, demonstrated under sensory neurons and the crosstalk of cells of osteoblastic lineage. Mechanisms by which cells of the osteoblast line provide this non-permissive environment for axons include paracrine-induced repulsion and loss of expression of neurotrophic factors. We have identified a drastic reduction in the production of NGF and BDNF and a stimulation of the expression of Sema3A, Wnt4 and Shh culminating in the advanced stage of OB differentiation. We noted a correlation between the Shh expression profile, the OB differentiation steps and the axonal repulsion mediated by OB. Blocking Shh activity and signaling has reversed the repulsive action of osteoblasts on sensory axons. Finally, to strengthen our model, we have localized the expression of Shh by osteoblasts in bone tissue. Overall, our results prove that the signaling profile associated with the osteoblast phenotype differentiation program can regulate the structuring of sensory innervation, and highlight Shh derived from osteoblasts as an essential player in this induced regulation. by signals.
Research Article: Journal of Physiotherapy & Physical Rehabilitation
Research Article: Journal of Physiotherapy & Physical Rehabilitation
Research Article: Journal of Physiotherapy & Physical Rehabilitation
Research Article: Journal of Physiotherapy & Physical Rehabilitation
Research Article: Journal of Physiotherapy & Physical Rehabilitation
Research Article: Journal of Physiotherapy & Physical Rehabilitation
Research Article: Journal of Physiotherapy & Physical Rehabilitation
Research Article: Journal of Physiotherapy & Physical Rehabilitation
Case Report: Journal of Physiotherapy & Physical Rehabilitation
Case Report: Journal of Physiotherapy & Physical Rehabilitation
Scientific Tracks Abstracts: Transplantation Technologies & Research
Scientific Tracks Abstracts: Transplantation Technologies & Research
Scientific Tracks Abstracts: Cancer Science & Therapy
Scientific Tracks Abstracts: Cancer Science & Therapy
Scientific Tracks Abstracts: Alternative & Integrative Medicine
Scientific Tracks Abstracts: Alternative & Integrative Medicine
Keynote: Cancer Science & Therapy
Keynote: Cancer Science & Therapy
Accepted Abstracts: Cancer Science & Therapy
Accepted Abstracts: Cancer Science & Therapy
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