Diffuse axonal injury (DAI) is characterized by delayed axonal disconnection. Although the effect of DAI on axonal pathology has been well documented, there is little information regarding the role of myelin in the pathogenesis of DAI. We used a modified Marmarou method to create a moderate DAI model in the adult rat and examined the corpus callosum and brainstem for myelin pathology and dynamic glial responses to DAI. During the first week following the DAI, Luxol Fast Blue staining and Western blot analysis for MBP showed significant loss of myelin in the corpus callosum and brainstem. Increased apoptosis of mature oligodendrocytes, as represented by its CC-1 marker, has been observed. Conversely, there was an increase in the number of Olig2 positive cells accompanied by hypertrophic microglia / macrophages and mild reactive astrocytes. Electron microscopy revealed degenerative axons in the corpus callosum and marked myelin abnormalities in the brainstem at the early stage of IAO. The regions of the cerebral rod presented intrusions of myelin or external projections with generalized delamination and collapse of the myelin, leading to the degeneration of the axons which accompany it. Our results show separate pathological processes involving the axon and myelin between the corpus callosum and the brainstem in DAI. The selective vulnerability of oligodendrocytes and subsequent demyelination can contribute to axonal degeneration of the brainstem. Defining the cause of continuous oligodendrocyte death and promoting myelin regeneration can be important targets for therapeutic AI interventions.
Editorials: Journal of Brain Research
Editorials: Journal of Brain Research
Letter to Editor: Journal of Brain Research
Letter to Editor: Journal of Brain Research
Editorial: Journal of Brain Research
Editorial: Journal of Brain Research
Research Article: Journal of Brain Research
Research Article: Journal of Brain Research
Research Article: Journal of Brain Research
Research Article: Journal of Brain Research
Scientific Tracks Abstracts: Pulmonary & Respiratory Medicine
Scientific Tracks Abstracts: Pulmonary & Respiratory Medicine
Scientific Tracks Abstracts: Cancer Science & Therapy
Scientific Tracks Abstracts: Cancer Science & Therapy
Posters-Accepted Abstracts: Cancer Science & Therapy
Posters-Accepted Abstracts: Cancer Science & Therapy
Posters & Accepted Abstracts: Journal of Hypertension: Open Access
Posters & Accepted Abstracts: Journal of Hypertension: Open Access
Posters & Accepted Abstracts: Journal of Bioengineering & Biomedical Science
Posters & Accepted Abstracts: Journal of Bioengineering & Biomedical Science
Journal of Brain Research received 2 citations as per Google Scholar report
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