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Medical Microbiology & Diagnosis

ISSN: 2161-0703

Open Access

Current Issue

Volume 9, Issue 1 (2020)

    Editorial Pages: 1 - 1

    Editorial Highlights for Journal of Medical Microbiology and Diagnosis

    Verma SC

    DOI: volume 9

    Without continual growth and progress, such words as improvement, achievement, and success have no meaning. Founded by Prof. Dr. Vikram Vermai (University of Minnesota, USA) Medical Microbiology and Diagnosis (ISSN: 2161-0703) is growing continuously. It is our pleasure to announce that during year 2019, all issues of volume 8 were published online on time and the print issues were also brought out and dispatched within 30 days of publishing the issue online. 

    Research Article Pages: 1 - 3

    Isolation and Identification of Microbes Causing Urinary Tract Infections from Hands and Mobile Phones

    Anum Liaquat-Ali, Umrah Imran, Afia Khatoon, Tahira Fareed, Sidra Afzal, Shaheen Sharafat and Nafisa Hassan-Ali

    Background: Hands and cell phones are the major source of cross- transmission of urinary tract infections. The
    aim of our study was to isolate, identify and evaluate Gram-negative bacteria from them.
    Method: This study was conducted in visiting area of Civil Hospital Karachi, Pakistan. Analysis was done by 50
    wet sterile cotton tipped swabs, 25 each from mobile phones and hands of their owners. Samples were transported
    in a Cary Blair transport media, Swabs were streaked on Nutrient agar, Blood agar and Macconkey agar. Organisms
    were identified by cultural, biochemical and microscopic characteristics. Frequency of bacterial species observed
    was evaluated.
    Results: A total of 25 samples were collected from cell phones, out of 3 samples were positive cell phones
    were collected from 50 persons having age between 28 to 43 and standard deviation 14.8052 ± 22.222 rang. Rate
    of bacteria found Serretia (24%), E. coli (24%), Pseudomonas (12%), Klebsiella (12%), Shigella (8%) and Proteus
    (12%). While cell phones were contaminated by E. coli, Klebsiella and Serretia with percentage of 54, 31 and 15
    respectively.
    Conclusion: Mobile phones and hands are reservoir of pathogenic organisms. Patient attendants in hospitals
    and visitors are more susceptible to nosocomial infections through exchange of mobile phones as their hands and
    mobile phones were contaminated by microbes causing urinary tract infections. To prevent infectious diseases
    hospitals should have enough outlets for washing hands with an ample supply of water. Hygienic practice of keeping
    hands clean and frequent mobile disinfection practices may help to break transmission cycle of pathogenic bacteria.

    Research Article Pages: 1 - 10

    Heterologous Expression of Staphylococcal Enterotoxin B and Its Suitability for Development of Field Based Diagnostic Test System

    Tirpude RJ

    Staphylococcal Enterotoxin B (SEB) is one of the potent enterotoxins produced by certain strains of Staphylococcus aureus bacteria. In the present context of its use as bio-terror agent, it is imperative to develop simple to use and rapid detection systems as well as therapeutic vaccines to detect SEB and protect against SEB intoxication. In the present study, recombinant staphylococcal enterotoxin B, produced as a NH2-terminal histidine hexamer fusion protein, expressed in heterologous expression system, was tested for its diagnostic potentials. His6-tagged SEB was immunogenic for IgG in mice and rabbits, either singly or in combination with adjuvant. Antigenic authenticity of the recombinant SEB to native SEB was confirmed by antibody-based capture detection assays across different host species. Using recombinant SEB as bound target, a sandwich ELISA was developed and utilized for detection of SEB in vitro and in vivo. The ELISA described here can be a useful tool for detection of SEB intoxication as well as infection due to SEB producing S. aureus bacteria. Data indicated potentials of recombinant SEB in diagnosis and vaccine development.

    Original Article Pages: 1 - 4

    Characterization of the ?Candida albicans Complex?: First Report of Candida africana in Tunisia

    Hana S, Latifa M, Camilia C and Boutheina J

    Background: New molecular studies tools have recently recognized Candida africana as a new atypical
    strain of the ‘C. albicans complex’ which consists previously of C. albicans and C. dubliniensis only. As currently
    available yeast identification tools fail to differentiate these species, their incidence is poorly understood in different
    geographical regions such as Tunisia. In this study, we analyzed the genetic diversity of the ‘C. albicans complex’.
    Materiel and methods: We selected 105 isolates of C. albicans and C. dubliniensis from oral and vaginal
    samples. These yeasts were phenotypically identified by chlamydosporulation on Agar Tween and Sunflower
    medium and biochemical tests using Vitek 2 Compact. To confirm this identification, the HWP1 gene was amplified
    by PCR using a single primer pair (CR-f/CR-r) for all isolates and sequenced for 16 strains.
    Results: Out of 105 oral and vaginal swabs, conventional identification tools revealed 67 C. albicans and 38
    C. dubliniensis. Screening of PCR products by electrophoresis gel highlights the presence of 64 C. albicans, 43 C.
    dubliniensis and one strain of C. africana. This is the first description of this species in Tunisia. Three cases of coinfection
    by C. albicans and C. dubliniensis were detected after molecular analysis.
    Conclusion: Candida albicans is the major selected yeast in human fungal pathologies. The study highlights
    the need to discriminate between this species and its related subgroups which greatly facilitates early initiation of
    pathogen-specific therapy.

Recommended Conferences

Infectious Diseases and Control

New York, USA

World Pathology Congress 2020

New York, USA
 
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