Jonathan Zintgraff

Servicio Bacteriología Clínica, Instituto Nacional de Enfermedades Infecciosas (INEI) – Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) “Dr. Carlos G. Malbrán”, Buenos Aires, Argentina

Publications

Research Article
À la carte, Streptococcus pneumoniae capsular typing. MALDI TOF mass spectrometry and machine-learning algorithms as complementary tools for the determination of PCV13 serotypes and the most prevalent NON PCV13 serotypes according to Argentina′s epidemiology
Author(s): Jonathan Zintgraff*, Florencia Rocca, Nahuel Sánchez Eluchans, Lucía Irazu, Maria Alicia Moscoloni, Claudia Lara and Mauricio Santos

Laboratory surveillance of Streptococcus pneumoniae serotypes is crucial for the successful implementation of vaccines to prevent invasive pneumococcal diseases. The reference method of serotyping is the Quellung reaction, which is labor-intensive and expensive. In the last few years, the introduction of MALDI-TOF MS into the microbiology laboratory has been revolutionary. In brief, this new technology compares protein profiles by generating spectra based on the m/z ratio. We evaluated the performance of MALDI-TOF MS for typing serotypes of S. pneumoniae isolates included in the PCV13 vaccine using a machine learning approach. We challenged our classification algorithms in “real time” with a total of new 100 isolates of S. pneumoniae from Argentinian nationwide surveillance. Our best approach could correctly identify the isolates with a sensitivi.. Read More»
DOI: 10.37421/ 2161-0703.2023.12.403

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