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Journal of Tissue Science and Engineering

Journal of Tissue Science and Engineering

ISSN: 2157-7552

Open Access

Geidy E Serrano

Dr, Banner Sun Health Research Institute, Arizona, United States

Publications
  • Research Article   

    Author(s): Geidy E Serrano*, Jessica E Walker, Anthony J Intorcia, Michael J Glass, Richard A Arce, Ignazio S. Piras, Joshua S Talboom, Courtney M Nelson, Brett D Cutler, Lucia I Sue, Lih-Fen Lue, Matthew Huentelman and Thomas G Beach

    Biochemical analysis of human brain tissue is typically done by homogenizing whole pieces of brain and separately characterizing the proteins, RNA, DNA, and other macromolecules within. While this has been sufficient to identify substantial changes, there is little ability to identify small changes or alterations that may occur in subsets of cells. To effectively investigate the biochemistry of disease in the brain, with its different cell types, we must first separate the cells and study them as phenotypically defined populations or even as individuals. In this project, we developed a new method for the generation of Whole Cell Dissociated Suspensions (WCDS) in fresh human brain tissue that could be shared as a resource with scientists to study single human cells or populations. Characterization of WCDS was done in paraffin-embedded sections stained with H&E, and by phenotyping w.. Read More»
    DOI: 10.37421/2157-7552.2024.15.400

    Abstract HTML PDF

Google Scholar citation report
Citations: 807

Journal of Tissue Science and Engineering received 807 citations as per Google Scholar report

Journal of Tissue Science and Engineering peer review process verified at publons

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