Cancer Science & Therapy

Dariusz Rakus

Publications

• Research Article
  Demethylation with 5-Aza-2²-deoxycytidine Affects Oxidative Metabolism in Human and Mouse Non-small Cell Lung Cancer Cells
  Author(s): Agnieszka J Sok, Agnieszka Gizak, Piotr Mamczur, Aleksandra Piotrowska, Agata Knapik, Jerzy Kolodziej, Piotr Dziegiel, Jacek R Wisniewski and Dariusz RakusAgnieszka J Sok, Agnieszka Gizak, Piotr Mamczur, Aleksandra Piotrowska, Agata Knapik, Jerzy Kolodziej, Piotr Dziegiel, Jacek R Wisniewski and Dariusz Rakus
  
  Background: Non-small cell lung cancer is the most common cause of death from cancer in the world. Here we demonstrate the effect of 5-aza-2’-deoxycytidine (5-dAza), which is one of the most promising anticancer drugs, on the growth and metabolism of mouse (KLN205) and human non-small cell lung cancer-derived (hNSCLC) cells. Results: In both cell lines we observed reduction of the cells viability and rate of their proliferation after 5-dAza treatment. These changes correlated with the lowering of ATP synthesis and lactate release from cells and with overproduction of reactive oxygen species. 5-dAza-treatment of lung cancer cells also induced a decrease of mitochondrial membrane potential and damage of mitochondrial network. These changes were partially reversed by rotenone treatme.. Read More»
  DOI: 10.4172/1948-5956.1000246

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