Cancer Science & Therapy

ISSN: 1948-5956

Open Access

Akulapalli Sudhakar

School of Medicine,
Director & Coordinator: Cell Signaling and Tumor Angiogenesis Laboratory, Boys Town National Research Hospital, 555 N, 30th Street, Omaha NE 68131

  • Research Article
    Validation of Different Systems for Tumstatin Expression and its in-vitro and in-vivo Activities
    Author(s): Chandra S Boosani, Ashok K Varma and Akulapalli SudhakarChandra S Boosani, Ashok K Varma and Akulapalli Sudhakar

    The aim of the present study is to identify an effective and efficient expression system for purification of recombinant antiangiogenic protein tumstatin. The sequence encoding carboxy-terminal non-collagenous domain of ?3 chain Type IV collagen, a3(IV)NC1 (tumstatin) was isolated from human placental tissue and cloned in three different expression vectors pET22b, pcBFT and pAcHLTA to express it in bacteria, mammalian and Sf-9 insect cells respectively. Expression and purification profiles of tumstatin were evaluated by coomassie staining and immunoblotting, and the efficiency was determined based on the yields of soluble protein. Our results indicate that, baculovirus expression system was efficient for scalable yields of soluble protein that could be purified in its biologically active form. This baculovirus expressed tumstatin was used to evaluate its anti-angiogenic and anti-tumar.. Read More»

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