Curcumin and quercetin are very poor water solubility, it could be these bio products useful and important in condition therapy related with cancer, oxidative stress and can used it to develop functional foods and to improve the foods shelf life. The objective was to prepare Curcumin and quercetin nanoparticles (CurNPs and QurNPs) give highly stable and soluble in water. The DPPH radical scavenging activity, HepG2 and HCT116 human cell lines (human cancer liver and colon) and antimicrobial of CurNPs and QurNPs were determined. The results showed that the CurNPs and QurNPs had the highest DPPH free radical scavenging activity at concentrate 1000 µg/ml were 94.91 and 98.11%, these results equal or nearly from ascorbic acid as at positive control at the same concentrate was 98.69%. CurNPs and QurNPs were significantly increased cell cytotoxicity, from the minimum to maximum concentrations ranging from 6.25 to100 µg/ml in HepG2 human cell lines (human cancer liver) which inhibition these cells at concentrate 100 µg/ml were 87.434 and 87.848% and the viability cells were 12.566 and 12.152%, IC50 = 31.15, 5.80, respectively. Meanwhile, the HCT116 human cell lines (human cancer colon), results found that the inhibition cells were 93.112 and 92.893% and the viability cells were 7.888, 7.1075 and IC50 = 22.65, 6.68, respectively. The highest inhibition zone in gram-positive bacteria than gram-negative bacteria was at 100µg/ml. Therefore, it could be recommended that drugs within nanoparticles enhance the entry of drugs within tumor tissues in vivo and antimicrobial by the enhanced retention and permeation effect.