Journal of Bioanalysis & Biomedicine

High-Stabilization of Biomolecules DNA-C, L on Magneto NPs

Abstract

Mansour Binandeh*, Farrokh Karimi and Sadegh Rostamnia

Objectives: The project is based on extensive studies on applied nanoparticles in biology and medicine.

Methodology: Fe₃O₄ Magnetic Nanoparticles (MNPs) with core/ shell structure was synthesized by a chemical-co-precipitation method. This work is done at 0.5 h to 48h with multi-times i.e., 20ng numbers of nanoparticles is provided regular with good morphology which was synthesized in <20 nm in size. Basic aim was used of nanoparticles in the absorption of biomolecules, DNA-L, C (linear, convoluted), which that detected by an Ultraviolet Spectrophotometer (UV-Vis).

Results: The results showed that absorption and diffusion of DNA-C or L at the surface of nanoparticles were 95% to 99% and 85% to 89% respectively (i.e. absorbance of DNA-C>DNA-L is) with rate of removing of on MNPs was >99%.

Conclusion: By examining the current experiment, 99% of DNAs can be stabilized on the surface of magnetic nanoparticles without any material loss. Finally, the pure DNA sample adsorbed on the surface of the magnetic nanoparticles can be isolated by an external magnetic field and a suitable method for transfer to the target cell that has a genetic defect can be modified and repaired.