Journal of Bioanalysis & Biomedicine

Evaluation of In Vitro Antidiabetic and Antioxidant Activities and Preliminary Phytochemical Screening of Leaf Extracts of Avicennia officinalis

Abstract

Swagat Kumar Das, Dibyajyoti Samantaray and Hrudayanath Thatoi

Avicennia officinalis is a medicinal mangrove plant and used in traditional folk medicine for treatment of various ailments. In the present study, the antidiabetic and antioxidant activities of petroleum ether and aqueous extracts of leaves of A. officinalis were evaluated. The antidiabetic activity was evaluated out by α-amylase and α-glucosidase inhibition study and antioxidant capacity by DPPH, superoxide and hydrogen peroxide scavenging activity of leaf extracts at various concentrations. The maximum antidiabetic activity was observed in 1.0 mg/ml of leaves extract of aqueous extracts. The maximum superoxide and hydrogen peroxide scavenging activity were exhibited by aqueous extracts with IC50 values of 0.27 and 0.17 mg/ml respectively and maximum DPPH scavenging activity was shown by petroleum ether extracts with IC50 value of 0.25 mg/ml. The qualitative and quantitative phytochemical analysis also showed presence of different phytochemicals including phenols, flavonoids and tannins which may be attributed to their antidiabetic and antioxidant activities. Thus, the present study gives a clear evidence that leaf extracts of A. officinalis possess both antidiabetic and antioxidant activities.