Cancer Science & Therapy

ISSN: 1948-5956

Open Access

Association of Butyric Acid Produced by Periodontopathic Bacteria with Progression of Oral Cancer


Yuji Miyazaki, Kentaro Kikuchi, Patricia González-Alva, Harumi Inoue, Yoshihiro Noguchi, Hozumi Tsuchiya, Joichiro Hayashi, Kitetsu Shin, Kuniyasu Ochiai and Kaoru Kusama

Objective: The association between periodontal disease and the risk of various human cancers including oral squamous cell carcinoma (OSCC) has been suggested. Butyric acid (BA), an extracellular metabolite from periodontopathic bacteria, plays an important role in the progression of periodontal disease. Recent studies have shown that podoplanin, a transmembrane glycoprotein, is expressed in various normal as well as neoplastic tissues. In this study, the effects of BA/sodium butyrate (NaB) on podoplanin expression, cell migration and epithelial-mesenchymal transition in OSCC cell lines were examined. Methods: Ca9-22, HSC-2, -3 and -4 cells were incubated with NaB, and then subjected to real-time RT-PCR, western blotting and scratch assay. Results: NaB increased the expression of podoplanin in HSC-2 and -3 cells and vimentin in Ca9-22 cells. Cell migration was promoted at a low concentration of NaB especially in HSC-3 cells, whereas it was inhibited in HSC-2 and -4 cells. Scratch assay of HSC-2, -3 and -4 revealed that cell migration was markedly inhibited by addition of a siRNA specific for podoplanin . Conclusion: BA/NaB increases podoplanin expression and cell migration in certain OSCC cell lines, suggesting that the progression of periodontal disease may promote the progression of OSCC via a podoplanin-dependent pathway


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