The inhibition effect and apoptotic mechanism of trichosanthin on SU-DHL-2 lymphoma cells

8^th Euro Global Summit on Cancer Therapy

November 03-05, 2015 Valencia, Spain

Ou Sha1, Ying-Jie Zhu2, Yue-Li Sun2 and Wen-Qi Jiang1,2

1Shenzhen University, People�s Republic of China
2Sun Yat-sen University Cancer Center, People�s Republic of China

Posters-Accepted Abstracts: J Cancer Sci Ther

Abstract :

In the past decades, many studies have revealed that Trichosanthin (TCS), a purified traditional Chinese medicine, exerts antitumor activities by inducing apoptosis in different tumor cell lines. However, little is known about the effects of TCS in lymphoma. The aim of this research was to study the in vitro effects of TCS on various lymphoma cells and the relative mechanisms. A variety of methods were employed including cell culture of 13 lymphoma cell lines, MTT assay, western blotting, flow cytometry, immunostaining, confocal, etc. Our results showed that TCS inhibited the proliferation of thirteen lymphoma cell lines in a dosedependent manner, while SU-DHL-2 cells were the most sensitive to TCS. SU-DHL-2 cells exposed to TCS led to cell arrest at the S to G2/M phase transition. Furthermore, flow cytometric analysis, Hoechst 33258 staining and western blotting disclosed that TCS-induced apoptosis of SU-DHL-2 cells in time- and concentration-dependent manners. Further studies showed that inhibition of caspase-8 and caspase-9 with pharmacological inhibitors reduced TCS-induced apoptosis. Caspase-8 inhibitor could completely reverse TCS-induced apoptosis. However, caspase-9 inhibitor only had partial reverse effect. Taken together, our findings firstly provided powerful evidences that TCS could induce apoptosis of SU-DHL-2 cells through both extrinsic and intrinsic pathways.

Biography :

Email: shaou@szu.edu.cn