Meral Koyuturk, Erengul Tokay, Elif Yaprak and Elif Guzel
Accepted Abstracts: J Cancer Sci Ther
In this study, the response of choriocarcinoma cells (JAR) to tumor necrosis factor-α (TNF-α) mediated oxidative stress was searched with the intention of offering new approaches for placental pathologies. Cells were cultured in DMEM containing 10% FBS in a humidified incubator at 37˚C with 5% CO2. Time and concentration depended preliminary experiments were performed. The effects of TNF-α (100ng) on NF-κB pathway, cell proliferation and apoptotic cell death and the effect of pentoxifylline (PTX) (10 mM) on TNF-α mediated NF-κB expression in choriocarcinoma cells were investigated by immunocytochemical tecniques. Cell proliferation rate was detected by PCNA, on the other hand determination of apoptotic cells was performed by anti-caspase-8. Staining intensities were semi-quantitatively evaluated by HSCORE. As a result of immunocytochemical staining, we determined that NF-κB expression was significantly changed at both nuclear and cytoplasmic levels according to the application time of TNF-α. TNF-α induced nuclear NF-κB levels was significantly lowered in choriocarcinoma cells when TNF-α was combined with PTX. In only PTX applied group, there was no nuclear NF-κB immunoreactivity in these cells.According to our results, PTX may be a potent therapeutic agent targeting NF-κB related signalling pathways.
Meral Koyuturk graduated from medical faculty on 1995 than she has completed her PhD in Histology and Embryology at the Istanbul Medical Faculty of Istanbul University. She attended Postdoctoral studies in Medical Faculties of Kadir Has University and Istanbul Bilim University. She has been working in the Cerrahpasa Medical Faculty of Istanbul University for the four last years. She has published book chapters and papers in reputed journals.
Cancer Science & Therapy received 3968 citations as per Google Scholar report
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