Flavonoids and coumarins from Prunus serotina Erth (capulín) show apoptosis in Hela cervical cancer cell line

5^th World Congress on Cancer Therapy

September 28-30, 2015 Atlanta, USA

Mart�­nez Vilchis Claudia Paulina, Silva Torres Rafael, Escart�­n Guti�©rrez Julio Rodrigo, Enr�­quez Rinc�³n Fernando and Figueroa Arredondo Paula

1Departamento de Posgrado e Investigaci�³n, Escuela Superior de Medicina del Instituto Polit�©cnico Nacional
2Laboratorio Nacional de Servicios Especializados LaNSE, Cinvestav-Zacatenco
3Lab de Anticonceptivos, Departamento de Farmaco

Posters-Accepted Abstracts: J Cancer Sci Ther

Abstract :

From the Cell Biology point of view, cancer is defined as the alteration of cell cycle, resulting of uncontrolled proliferation and inhibition of apoptosis. In Mexico, breast and cervical cancer stand out with high impact and mortality rates. Since cervical cancer is still a disease with high incidence rate and mortality in Mexico there is much interest in developing novel treatments for chemotherapy based in Mexican plants. Prunus serotina Erth is an example of Mexican flora and fauna species remaining to be studied, therefore, an integral research protocol using Prunus serotina Erth fruits (locally named capul�­n), is currently being conducted in our labs. An ethanolic extract from the fruit was applied to an in vitro model based on HeLa tissue culture cells. Since secondary metabolites from Prunus serotina Erth such as flavonoids and coumarins may inhibit proliferation or directly act as cytotoxics, inducing apoptosis or necrosis in tumor cells, we studied cell viability after treatment of Hela cells with extracts at different concentrations. Fractions containing coumarins and flavonoids revealed a cytotoxic effect, initially observed by inverted optic microscopy. Furthermore, when cell death was later estimated by flow cytometry techniques, apoptotic cell death was discovered.

Biography :

Email: paula_figueroa@outlook.com