Combination therapy of natural products with conventional anticancer drugs

9^th Indo Global Summit on Cancer Therapy

November 02-04, 2015 Hyderabad, India

Sandip Pawar1, Soumen Roy2, Sandeepan Mukherjee2, Abhay Chawdhary2 and Debjani Dasgupta3

1The Institute of Science, India 2Haffkine Institute of Training, Research and Testing, India 3D Y Patil University, India

Posters-Accepted Abstracts: J Cancer Sci Ther

Abstract :

Cervical cancer is the most common gynecological malignancy and a major threat to the health of women. It represents the second most common cancer worldwide. Botanicals rich in polyphenols in combination with chemotherapeutic drugs can be used effectively for the development of promising anti-cervical cancer therapies. The aim of the present work is to evaluate growth inhibitory effects of aqueous and ethanolic extracts of H. mystax, N. nimmoniana and O. tenuiflorum on KB (cervical cancer) cell line alone and in combination with camptothecin. Plant extracts were quantitatively determined for phenolic acids using RP-HPLC. Cytotoxicity analysis of extracts alone and in combination was performed by MTT assay. Selective toxicity of plant extracts were checked using normal cell line. The isobologram and combination index (CI) method of Chou-Talalay were used to evaluate the interactions between extracts and drug. Synergistic combinations induced cancer cell death was studied by Flow cytometry. Highest amount of phenolic acids present were vanillin in H. mystax and O. tenuiflorum and catechol in N. nimmoniana. Ethanol extracts of H. mystax and O. tenuiflorum and N. nimmoniana showing high anticancer activity were subjected to combination study. Six combinations were prepared using constant drug ratio. Combination treatment on KB cells for 24 hours showed synergistic growth inhibitory effect at medium and high dose levels (IC75 and IC90); whereas, 48 hours exposure showed synergistic growth inhibitory effect at all dose levels (IC50-IC90). The dose reduction level was different for each combination. From the present study it could be concluded that, camptothecin when combined with Ocimum tenuiflorum produced a synergistic effect in inhibiting growth of cervical cancer cell line. Therefore, conventional chemotherapeutic drugs when combined with natural products may help to minimize the dose related toxicity of chemotherapeutics.

Biography :

Email: drdebjanid@dypatil.edu