In this study, experiments are designed to research the consequences of ultrasonic flowers of zinc nanoparticles on the properties of petroleum flow. The rheological and thermal properties of the petroleum containing nanoparticles were investigated in vitro. The nanoparticles were prepared by ultrasonic method in petroleum . Experiments were administered during a hot tube for plain oil and nanoparticles containing flowers of zinc. The effect of temperature changes, addition of nanoparticles and tube length on the values of friction coefficient, velocity, conductivity coefficient, overall heat transfer coefficient, thermal diffusion coefficient and kinematic viscosity were investigated. For nano oil (containing 1wt.% Zinc oxide) Reynolds number decreased to 0.99 initial value, Prandtl number decreased to 0.951 initial value, Peclet number increased to 0.94 initial value.