Journal of Cytology & Histology

ISSN: 2157-7099

Open Access

From 2D Cell Phenotypes to 3D Live High-content Imaging: New Ways to Windows


Kubatiev AA, Zurina IM, Kosheleva NV, Gorkun AA, Saburina IN and Repin VS

Researches in the field of cellular and molecular mechanisms of cell variability, based on phenotypic interactions, remain one of the most central and promising problems in modern biology. The current paper proposes a possible model for studying "variability windows" and cell phenotype reprogramming using different somatic cell types (epithelial and mesenchymal cells) as well as different cell culture systems - 2D monolayer, 3D single spheroids and microtissues accompanied with up-to-date live time-lapse microscopy analysis.


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