Veterinary Science & Technology

Pei-Yu Alison Lee

Publications

• Research Article
  Rapid Detection of Type 2 Porcine Reproductive and Respiratory Syndrome Virus by a Duplex Reverse Transcription Insulated Isothermal PCR on a Field-Deployable System
  Author(s): Hung-Chih Kuo, Dan-Yuan Lo, Chiu-Lin Chen, Chien-Hsien Lee, Yu-Han Shen, Yung-Long Tsai, Pei-Yu Alison Lee and Hsiao-Feng Grace ChangHung-Chih Kuo, Dan-Yuan Lo, Chiu-Lin Chen, Chien-Hsien Lee, Yu-Han Shen, Yung-Long Tsai, Pei-Yu Alison Lee and Hsiao-Feng Grace Chang
  
  Porcine reproductive and respiratory syndrome virus (PRRSV) is an important porcine pathogen globally. Reverse transcription-polymerase chain reaction (RT-PCR) for PRRSV detection is an important tool for disease management and control. Clinical sensitivity of RT-PCR for PRRSV detection is compromised to a certain degree by the high genetic diversity in the PRRSV genome. A duplex RT-insulated isothermal PCR (RT-iiPCR) for the North America lineage of PRRSV (PRRSV-NA) has been developed by targeting both ORF6 and ORF7 to increase test inclusivity. In this study, its limit of detection 95% was determined to be about 5 genome equivalents per reaction by testing a serial dilution of in-vitro transcribed RNA. The PRRSV-NA duplex RT-iiPCR was compared with an ORF7 real-time RT-PCR (rRT-PCR) published previously for the evaluation of analytical and clinical performance. Both tests did not re.. Read More»
  DOI: 10.4172/2157-7579.1000415

  Abstract PDF