Journal of Bioanalysis & Biomedicine

ISSN: 1948-593X

Open Access

Paul W. Sylvester

College of Pharmacy, 700 University Avenue,
Monroe, LA 71209-0470

  • Research Article
    Mechanisms Mediating the Synergistic Anticancer Effects of Combined ?Ž?³-Tocotrienol and Celecoxib Treatment
    Author(s): Amit B. Shirode and Paul W. SylvesterAmit B. Shirode and Paul W. Sylvester

    Aim: To characterize the intracellular signaling mechanisms mediating the synergistic anticancer effects of combined ?-tocotrienol and celecoxib treatment in neoplastic +SA mouse mammary epithelial cells in vitro. Methods: +SA mammary tumor cells in different treatment groups were maintained in serum-free defined media containing 10ng/ml EGF as a mitogen and exposed to various doses of ? -tocotrienol and celecoxib alone or in combination. After a 96 hr culture period, cells were collected and whole cell lysates were subjected to Western blot analysis to determine treatment effects on intracellular signaling proteins associated with EGF-dependent mitogenesis and survival, and prostaglandin synthesis and responsiveness. Results: Treatment with high doses of ?-tocotrienol or celecoxib alone inhibited Akt activation and downstream signaling and NF?B activation. Similar treatment with .. Read More»
    DOI: 10.4172/1948-593X.1000036

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