Saudi Arabia						                            
                            
						
 Research Article
												Estimation of Swertiamarin in Enicostemma Littorale and Marketed Formulations Using HPLC-UV Method 						
Author(s): Prawez Alam, Mohammad Ali, Rahul Singh and Faiyaz ShakeelPrawez Alam, Mohammad Ali, Rahul Singh and Faiyaz Shakeel             
						
												
				 A  simple,  economic,  reproducible,  robust  and  precis e HPLC  method  for  estimation  of  swertiamarin  in  both  60 % methanolic extract of  Enicostemma littorale  and  mar- keted formulations was developed and validated in p resent investigation.  The  mobile  phase  composed  of  methano l and  water  (90:10  %  v/v),  gave  a  sharp  and  well-defi ned peak of swertiamarin at the retention time of 10.15  ± 1.52 min. The limit of detection (LOD) and limit of quan tifica- tion (LOQ) were 17.25 and 56.92  μ g/ml respectively. The proposed method with high degree of precision and a ccu- racy  was  employed  for the  estimation  of  swertiamari n  in methanolic  extract  and  in  marketed  formulation... Read More»
				  
												DOI:
												 10.4172/1948-593X.1000005 
																	  
Journal of Bioanalysis & Biomedicine received 3099 citations as per Google Scholar report