Cancer Science & Therapy

Christopher N. Parris

Publications

• Research Article
  Radiosensitivity of Human Breast Cancer Cell Lines Expressing the Breast Tumor Kinase (Brk)
  Author(s): Bourton EC, Hussain H, Plowman PN, Amanda J. Harvey and Christopher N. ParrisBourton EC, Hussain H, Plowman PN, Amanda J. Harvey and Christopher N. Parris
  
  The breast tumor kinase (Brk) is over-expressed in 80% of all breast cancers and we sought to determine the influence of this oncogene on radiation sensitivity in breast cancer cell lines. Since radiotherapy is a routinely used method of treatment for early and intermediate stage breast cancer, the alteration of clinical radiosensitivity in breast cancer by an oncogene over-expression would have important implications in radiotherapy management. To address this question, we conducted an in vitro study of radiation sensitivity in two breast cancer cell lines, MDA-MB-157 and MDA-MB-468 transfected with cDNA constructs to over-express the following genes: Brk wild type (WT); Brk with an inactivating mutation in the kinase domain (KM) and vector only. Gamma H2AX foci assays by imaging flow cytometry were used to measure DNA double strand break (DSB) repair aft.. Read More»
  DOI: 10.4172/1948-5956.1000331

  Abstract PDF